Autologous Cord Blood Infusions Are Safe and Feasible in Young Children with Autism Spectrum Disorder: Results of a Single‐Center Phase I Open‐Label Trial
Geraldine Dawson, Jessica M. Sun, Katherine S. Davlantis, Michael Murias, Lauren Franz, Jesse Troy, Ryan Simmons, Maura Sabatas-DeVito, Rebecca Durham, Joanne Kurtzberg, STEM CELLS Translational Medicine
This phase I study demonstrates that it is safe and feasible to perform autologous umbilical cord blood infusions in young children with autism spectrum disorder and identifies several promising outcome measures for use in future trials.
Read the related CNN article here.
Emilie Dambroise et al., STEM CELLS
This study provides an integrated view of neuroepithelial-like cells in the adult fish brain. These results challenge the widely-accepted view that adult neurogenesis relies on radial glia and will fuel discussions on the nature of extensive cell proliferation observed in these animals. As never reported before in vertebrates, the researchers show cells paused in G2, thereby pointing to novel pathways of quiescence control in stem cells, including for example the DNA repair machinery.
Donor‐Dependent and Other Nondefined Factors Have Greater Influence on the Hepatic Phenotype Than the Starting Cell Type in Induced Pluripotent Stem Cell Derived Hepatocyte‐Like Cells
James A. Heslop et al., STEM CELLS Translational Medicine
Current protocols for producing hepatocyte-like cells (HLCs) do not produce fully mature hepatocytes. Previous reports have suggested an improved phenotype may be yielded by using the incomplete resetting of the epigenome during induced pluripotent stem cell (iPSC) generation. We report the first comparison of iPSCs derived from human isogenic hepatocytes and fibroblasts for producing HLCs. We find little significant difference between iPSC-HLCs derived from hepatocytes and the easier to access and reprogram fibroblasts—an important finding for current and future studies which require patient specific (i.e., for transplantation) or disease/genotype specific phenotypes.
High Aldehyde Dehydrogenase Activity Identifies a Subset of Human Mesenchymal Stromal Cells with Vascular Regenerative Potential
Stephen E. Sherman et al., STEM CELLS
Applying core stem cell concepts using aldehyde dehydrogenase (ALDH) as a conserved marker of primitive progenitor cells, mesenchymal stromal cells (MSCs) appear to have a functional hierarchy where vascular regenerative potential is diminished as ALDH activity is reduced with cell differentiation. Purified ALDHlo and ALDHhi MSCs demonstrated clear secretory differences after purification, which correlated with endothelial cell activation in vitro and enhanced stimulation of proangiogenic processes in vivo. Thus, ALDHhi MSCs represent a proangiogenic MSC subset with regenerative potential applicable to the development of cell therapies to augment therapeutic revascularization.
Efficacy and Safety of Immuno-Magnetically Sorted Smooth Muscle Progenitor Cells Derived from Human-Induced Pluripotent Stem Cells for Restoring Urethral Sphincter Function
Yanhui Li et al., STEM CELLS Translational Medicine
This study provides a promisingly clinical-scale method for enriching committed cells from Human-induced pluripotent stem cells (hiPSCs) differentiation. Magnetic-activated cell sorting (MACS)-sorted smooth muscle progenitor cells (pSMCs) were heterogenous with negligible undifferentiated cells after in vitro directed differentiation. This cell population showed long-term tissue integration with no in vivo tumor formation. Furthermore, MACS-sorted pSMCs efficiently induced the remodeling of extracellular matrix of lower urinary tract and promoted functional recovery of the damaged urethra sphincter in a SUI animal model. These findings should facilitate translation of hiPSC-based cell therapy to clinical applications for smooth muscle regeneration.
Video abstract from Dr. Cooke, et al. on his recently published STEM CELLS paper entitled, "Retinoic Acid Inducible Gene 1 Protein (RIG1)-like Receptor Pathway is Required for Efficient Nuclear Reprogramming." Read the paper here.
Video abstract from Drs. Cox, Hetz, Liao, Aertker, Ewing-Cobbs, Juranek, Savitz, Jackson, Romanowska-Pawliczek, Triolo, Dash, Pedroza, Lee, Worth, Aisiku, Choi, Holcomb, and Kitagawa on their recently published STEM CELLS paper entitled, "Treatment of Severe Adult Traumatic Brain Injury Using Bone Marrow Mononuclear Cells." Read the paper here.Video Library