Atsushi Ueda et al., STEM CELLS
Ets-related transcription factor GA-binding protein alpha (GABPα), which is encoded by Gabpa, is expressed in a variety of cell types and is involved in cellular functions such as cell cycle regulation, apoptosis, and differentiation. This research analyzed the disruption of Gabpa and the proliferation of Gabpa-null ESCs that was drastically repressed. The repressed proliferation of Gabpa-null embryonic stem cells (ESCs) was recovered by artificially forced expression of GABPα. Expression analysis showed that p53 mRNA levels were comparable; however, p53 target genes were upregulated and cell cycle-related genes were downregulated in Gabpa-null ESCs. Interestingly, p53 and cleaved Caspase3 expressions were enhanced in the cells and reduced proliferation as well as cell death of Gabpa-null ESCs were rescued by either transfection of p53 RNAi or treatment of the p53 inhibitor pifithrin-α. These results suggest that GABPα inhibits the accumulation of p53 and is involved in the proliferation and survival of ESCs.
Sean V. Murphy et al., STEM CELLS Translational Medicine
The early and effective treatment of wounds is vital to ensure proper wound closure and healing with appropriate functional and cosmetic outcomes. This study confirms the efficacy of the amnion membrane as a wound treatment/dressing, and overcomes many of the limitations associated with using fresh, cryopreserved, or dehydrated tissue.
The Wnt5a Receptor, Receptor Tyrosine Kinase-Like Orphan Receptor 2, Is a Predictive Cell Surface Marker of Human Mesenchymal Stem Cells with an Enhanced Capacity for Chondrogenic Differentiation
Sally C. Dickinson et al., STEM CELLS
Mesenchymal stem cells (MSCs) can be turned into cartilage-forming cells. However, these stem cells vary from one donor to the other in their capacity to form cartilage, and they lose this capacity altogether if they are grown for too long in the laboratory. A marker protein on the surface of the stem cells might be used to predict which are best able to make cartilage. This research details the growth of MSCs clones and revealed clones that are good for making cartilage and those are very poor at doing so. Through comparison of these clones, a protein was identified, ROR2, that is present at higher levels on those MSCs that are very good at making cartilage. This new marker may help to ensure a more effective cell therapy for cartilage injuries.
Cryopreserved Off-the-Shelf Allogeneic Adipose-Derived Stromal Cells for Therapy in Patients with Ischemic Heart Disease and Heart Failure—A Safety Study
Jens Kastrup et al., STEM CELLS Translational Medicine
This first-in-human study of an off-the-shelf cryopreserved Cardiology Stem Cell Centre adipose-derived stromal cell product from healthy donors demonstrated safety, feasibility, and a tendency toward clinical efficacy in ten patients with ischemic heart disease and heart failure. The presence of a ready-to-use cryo-stored cell product will eliminate many of the logistic barriers in disseminating cell therapy to many patient groups and will also reduce the treatment costs.
Video abstract from Dr. Cooke, et al. on his recently published STEM CELLS paper entitled, "Retinoic Acid Inducible Gene 1 Protein (RIG1)-like Receptor Pathway is Required for Efficient Nuclear Reprogramming." Read the paper here.
Video abstract from Drs. Cox, Hetz, Liao, Aertker, Ewing-Cobbs, Juranek, Savitz, Jackson, Romanowska-Pawliczek, Triolo, Dash, Pedroza, Lee, Worth, Aisiku, Choi, Holcomb, and Kitagawa on their recently published STEM CELLS paper entitled, "Treatment of Severe Adult Traumatic Brain Injury Using Bone Marrow Mononuclear Cells." Read the paper here.Video Library