Featured in STEM CELLS

Skip slideshow

Quality Control of Keratinocyte Stem Cells Cultures

This deep learning-based automated cell tracking (DeepACT) technology rapidly analyzed the motion of keratinocytes and provided the collective motion dynamics in cultured keratinocytes, which enabled the quantitative evaluation of keratinocyte dynamics in response to changes in culture conditions.

Microglia in MS Gray Matter

Understanding microglial responses to gray matter-specific cues will shed light on multiple sclerosis pathological processes and guide therapeutic strategies targeting the gray matter for neuroprotection—an unmet need to avoid and treat disability.

Transplantation of PSC-Derived Photorecpters

In the present study, the authors have advanced the field by assessing the full range of light responses that develop following transplantation of pluripotent stem cell-derived cone photoreceptors in a mouse model of advanced photoreceptor dystrophy.

MSC-Mediated Immune Regulation in T2DM Therapy

This review reports on the plasticity and underlying mechanisms of MSC-mediated immune modulation and further uncovers the therapeutic potential of MSCs for T2DM.

MSCs Regulate ILC3s Via ICOS-ICOSL Activated Tregs

The present study demonstrated for the first time that induced pluripotent stem cell-derived MSCs (iPSC-MSCs) are able to inhibit ILC2 functions via the assistance of regulatory T (Treg) cells.

 

 

Browse Articles

Open access

State‐of‐play for cellular therapies in cardiac repair and regeneration

Ramana Vaka, Darryl R. Davis,
  •  27 August 2021

Abstract

Cardiovascular disease is the primary cause of death around the world. For almost two decades, cell therapy has been proposed as a solution for heart disease. In this article, we report on the “state-of-play” of cellular therapies for cardiac repair and regeneration.
Free to Read

Transient receptor potential vanilloid 4 as a regulator of induced pluripotent stem cell chondrogenesis

Vincent P. Willard, Holly A. Leddy, Daniel Palmer, Chia-Lung Wu, Wolfgang Liedtke, Farshid Guilak,
  •  24 August 2021

Abstract

As induced pluripotent stem cells (iPSCs) undergo chondrogenesis, Trpv4 expression mirrored the expression of other chondrogenic markers (eg, Sox9, Acan, and Col2a1). Using a Col2a1-GFP reporter, green fluorescent protein positive (GFP+) cells showed increased chondrogenesis and expression of Trpv4. Daily activation of transient receptor potential vanilloid 4 (TRPV4) significantly increased cartilaginous matrix production in GFP+ cells. These findings suggest that TRPV4 serves as a marker as well as a regulator of iPSC chondrogenesis.
Open access

Aryl hydrocarbon receptor controls skin homeostasis, regeneration, and hair follicle cycling by adjusting epidermal stem cell function

Eva María Rico-Leo, Luis Francisco Lorenzo-Martín, Ángel Carlos Román, Xosé Ramón Bustelo, Jaime María Merino, Pedro María Fernández-Salguero,
  •  23 August 2021

Abstract

Aryl hydrocarbon receptor (AhR) is required for skin homeostasis and hair growth. AhR depletion in keratinocytes and dermal fibroblasts compromises skin regeneration likely because of reduced epidermal stem cells (EpdSCs) numbers. Reconstitution assays suggest a cell autonomous role for AhR in the epidermis. Signaling networks controlling skin homeostasis are AhR regulated in EpdSCs. AhR modulation by physiological ligands may represent a strategy to treat skin pathology.
Open access

Epigenetic regulation of neural stem cells: The emerging role of nucleoporins

Claudia Colussi, Claudio Grassi,
  •  16 August 2021

Abstract

Cartoon illustrating the contribution of Nups to epigenetic mechanisms in stem cells. (1) Transcription. (2) Heterochromatin formation. (3) Silencing. (4) Topological associated domain formation. (5) miRNA biogenesis and transport. (6) Protein SUMOylation.

Single‐cell transcriptomic sequencing analyses of cell heterogeneity during osteogenesis of human adipose‐derived mesenchymal stem cells

Rongmei Qu, Kai He, Tingyu Fan, Yuchao Yang, Liyao Mai, Zhiwei Lian, Zhitao Zhou, Yan Peng, Asmat Ullah Khan, Bing Sun, Xiaolan Huang, Jun Ouyang, Xinghua Pan, Jingxing Dai, Wenhua Huang,
  •  4 August 2021

Abstract

We investigated chemically induced osteogenesis from human adipose mesenchymal stem cells, human adipose mesenchymal stem cells (hAMSCs) using single-cell RNA-sequencing (scRNA-seq). We identified the cellular heterogeneity and their molecular and network regulatory pattern of osteogenic differentiation.
Open access

Three‐dimensional migration of human amniotic fluid stem cells involves mesenchymal and amoeboid modes and is regulated by mTORC1

Margit Rosner, Markus Hengstschläger,
  •  31 July 2021

Abstract

This study on human amniotic fluid stem cells (hAFSCs) provides the first demonstration that human stem cells exhibit mTORC1-dependent invasive capacity and can concurrently make use of mesenchymal and amoeboid 3D cell migration modes. These results represent an important step toward the full biological characterization of fetal human stem cells with relevance to developmental research and stem cell-based therapy.

Downregulation of augmenter of liver regeneration impairs the therapeutic efficacy of liver epithelial progenitor cells against acute liver injury by enhancing mitochondrial fission

Yuan Dong, Weining Kong, Wei An,
  •  26 July 2021

Abstract

Knockdown of augmenter of liver regeneration (ALR) in liver epithelial progenitor cells (LEPCs) impaired cell survival and resulted in excessive mitochondrial fission partially by activation of dynamin-related protein 1 (Drp1) phosphorylation at S616. Overexpression of ALR in LEPCs significantly inhibited Drp1 phosphorylation, maintained the mitochondrial integrity and the preservation of adenosine triphosphate contents. Consequently, the ALR-bearing LEPCs transplanted into ALI mice exhibited substantially greater homing ability to the injured liver through SDF-1/CXCR4 axis than that of LEPCs-lacking ALR.

Impaired generation of mature neurons due to extended expression of Tlx by repressing Sox2 transcriptional activity

Yu Wang, Bin Li, Lianwei Dong, Weibing Duan, Anna Neuerburg, Han Zhang, Xijuan Jiang, Rui Shao, Yan Zhu, Dagmar Bock, Erwei Liu, Hong Wang, Yunsha Zhang, Yifan Dai, Haiyuan Yang, Ying Wang,
  •  16 July 2021

Abstract

Without shutting-off on schedule, extended expression of Tailless (Tlx) in neural stem cells (NSCs) and their progenies prevents NSCs from neural maturation by repressing Sox2 transcriptional activity.
Open access

Human multipotent adult progenitor cells effectively reduce graft‐vs‐host disease while preserving graft‐vs‐leukemia activity

Leland Metheny, Saada Eid, Patiwet Wuttisarnwattana, Jeffery J. Auletta, Chen Liu, Alana Van Dervort, Conner Paez, ZhengHong Lee, David Wilson, Hillard M. Lazarus, Robert Deans, Wouter Vant Hof, Yiouli Ktena, Kenneth R. Cooke,
  •  13 July 2021

Abstract

Bio-redistribution of multipotent adult progenitor cells from the lung and to the liver and spleen of allogeneic blood and marrow transplantation recipient mice as captured by 3D, computer-generated imagery.
Open access

Bone‐forming perivascular cells: Cellular heterogeneity and use for tissue repair

Jiajia Xu, Yiyun Wang, Mario A. Gomez-Salazar, Ginny Ching-Yun Hsu, Stefano Negri, Zhao Li, Winters Hardy, Lijun Ding, Bruno Peault, Aaron W. James,
  •  12 July 2021

Abstract

Mesenchymal progenitor cells in the tunica adventitia have a hierarchy of differentiation and proliferation potential. On top of the hierarchy, platelet-derived growth factor receptor (PDGFR)α+ and aldehyde dehydrogenase (ALDH)High cells show a bipotent differentiation potential into osteogenic and adipogenic cell lineages with high proliferative rate. Conversely, CD10 and CD107a expression separate osteogenic progenitors from adipogenic progenitors, respectively. Moreover, osteoprogenitors can transition into adipogenic phenotype.
more >
free access

A protein‐centric view of in vitro biological model systems for Schizophrenia

Abinaya Chandrasekaran, Pia Jensen, Fadumo A. Mohamed, Madeline Lancaster, Michael E. Benros, Martin R. Larsen, Kristine K. Freude,
  •  25 August 2021

Abstract

Schematic diagram of stem-cell derived 2D SCZ culture models (left column) vs 3D SCZ brain organoid models (middle column) to engineer neural networks for translational medicine. SCZ patient cells are reprogrammed towards human induced pluripotent stem cells (hiPSC). Derived 3D brain organoids from hiPSCs serve as a model for further investigations. The application of proteomics and Post-translational Modification specific proteomics (PTMomics) on 3D organoids could contribute to a wealth of information regarding the underlying mechanisms of the diseases and cell responses to in vivo settings (right column).
Open access

Spatiotemporal Extracellular Matrix Modelling (StEMM) for In Situ Cell Niche Studies

Kim Olesen, Sergey Rodin, Wing Cheung Mak, Ulrika Felldin, Cecilia Österholm, Andreas Tilevik, Karl-Henrik Grinnemo,
  •  21 August 2021

Abstract

Spatiotemporal Extracellular Matrix Modeling (StEMM) facilitates the identification of cell-niches by combining decellularized whole organ sections and recellularization with a new algorithm for automatic generation of density maps and cluster-analysis for identification of regions of interest.
Open access

Prostacyclin is an endosteal bone marrow niche component and its clinical analog iloprost protects hematopoietic stem cell potential during stress

Joshua Tay, Valerie Barbier, Falak M. Helwani, Gareth R. Price, Jean-Pierre Levesque, Ingrid G. Winkler,
  •  14 July 2021

Abstract

Prostacyclin/prostaglandin I2 (PGI2) is a novel hematopoietic stem cell (HSC) regulatory factor enriched at the endosteum, synthesized by its synthase PTGIS expressed mainly on osteoblasts (OB), followed by mesenchymal stromal cells (MSC) and endothelial cells (EC). Ex vivo and in vivo treatment with PGI2 analogues enhance HSC long-term competitive repopulation potential and protect reconstituting HSC from stress.
free access

Organoid Technology: Current Standing and Future Perspectives

Laleh Shariati, Yasaman Esmaeili, Shaghayegh Haghjooy Javanmard, Elham Bidram, Abbas Amini,
  •  30 March 2021

Abstract

Organoid generation and therapeutic potential
more >
free access

Concise Review: MSC‐Derived Exosomes for Cell‐Free Therapy

Donald G. Phinney, Mark F. Pittenger,
  • STEM CELLS
  •  851-858
  •  10 March 2017
free access

Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose Tissue

Susanne Kern, Hermann Eichler, Johannes Stoeve, Harald Klüter, Karen Bieback Ph.D.,
  • STEM CELLS
  •  1294-1301
  •  2 January 2009
free access

Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing

Giselle Chamberlain, James Fox, Brian Ashton, Jim Middleton Ph.D.,
  • STEM CELLS
  •  2739-2749
  •  2 January 2009
free access

Concise Review: The Surface Markers and Identity of Human Mesenchymal Stem Cells

Feng-Juan Lv, Rocky S. Tuan, Kenneth M.C. Cheung, Victor Y.L. Leung,
  • STEM CELLS
  •  1408-1419
  •  28 February 2014
free access

Mesenchymal Stem Cells Enhance Wound Healing Through Differentiation and Angiogenesis

Yaojiong Wu M.D., Ph.D., Liwen Chen, Paul G. Scott, Edward E. Tredget M.D., M.Sc., FRCSC,
  • STEM CELLS
  •  2648-2659
  •  2 January 2009
free access

Exosome‐Mediated Transfer of miR‐133b from Multipotent Mesenchymal Stromal Cells to Neural Cells Contributes to Neurite Outgrowth

Hongqi Xin, Yi Li, Ben Buller, Mark Katakowski, Yi Zhang, Xinli Wang, Xia Shang, Zheng Gang Zhang, Michael Chopp,
  • STEM CELLS
  •  1556-1564
  •  17 May 2012
free access

Intra‐Articular Injection of Mesenchymal Stem Cells for the Treatment of Osteoarthritis of the Knee: A Proof‐of‐Concept Clinical Trial

Chris Hyunchul Jo, Young Gil Lee, Won Hyoung Shin, Hyang Kim, Jee Won Chai, Eui Cheol Jeong, Ji Eun Kim, Hackjoon Shim, Ji Sun Shin, Il Seob Shin, Jeong Chan Ra, Sohee Oh, Kang Sup Yoon,
  • STEM CELLS
  •  1254-1266
  •  21 January 2014
free access

Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair—Current Views

Donald G. Phinney Ph.D., Darwin J. Prockop,
  • STEM CELLS
  •  2896-2902
  •  2 January 2009
Open access

Concise Review: Evidence for CD34 as a Common Marker for Diverse Progenitors

Laura E. Sidney, Matthew J. Branch, Siobhán E. Dunphy, Harminder S. Dua, Andrew Hopkinson,
  • STEM CELLS
  •  1380-1389
  •  4 February 2014
free access

MiR‐133b Promotes Neural Plasticity and Functional Recovery After Treatment of Stroke with Multipotent Mesenchymal Stromal Cells in Rats Via Transfer of Exosome‐Enriched Extracellular Particles

Hongqi Xin, Yi Li, Zhongwu Liu, Xinli Wang, Xia Shang, Yisheng Cui, Zheng Gang Zhang, Michael Chopp,
  • STEM CELLS
  •  2737-2746
  •  30 April 2013
more >
free access

Concise Review: MSC‐Derived Exosomes for Cell‐Free Therapy

Donald G. Phinney, Mark F. Pittenger,
  • STEM CELLS
  •  851-858
  •  10 March 2017
free access

A Review of the Costs, Cost‐Effectiveness and Third‐Party Charges of Bone Marrow Transplantation

Ilana L. Westerman, Charles L. Bennett,
  • STEM CELLS
  •  312-319
  •  May 1996
free access

Concise Review: The Surface Markers and Identity of Human Mesenchymal Stem Cells

Feng-Juan Lv, Rocky S. Tuan, Kenneth M.C. Cheung, Victor Y.L. Leung,
  • STEM CELLS
  •  1408-1419
  •  28 February 2014
free access

Concise Review: Mesenchymal Stem Cells: From Roots to Boost

Anna Andrzejewska, Barbara Lukomska, Miroslaw Janowski,
  • STEM CELLS
  •  855-864
  •  12 April 2019

Abstract

Mesenchymal stem cells: from isolation to transplantation.
free access

Intra‐Articular Injection of Mesenchymal Stem Cells for the Treatment of Osteoarthritis of the Knee: A Proof‐of‐Concept Clinical Trial

Chris Hyunchul Jo, Young Gil Lee, Won Hyoung Shin, Hyang Kim, Jee Won Chai, Eui Cheol Jeong, Ji Eun Kim, Hackjoon Shim, Ji Sun Shin, Il Seob Shin, Jeong Chan Ra, Sohee Oh, Kang Sup Yoon,
  • STEM CELLS
  •  1254-1266
  •  21 January 2014
free access

Distinct Roles for Wnt‐4 and Wnt‐11 During Retinoic Acid‐Induced Neuronal Differentiation

Carina Elizalde, Victor M. Campa, Mercedes Caro, Karin Schlangen, Ana María Aransay, Maria dM Vivanco, Robert M. Kypta,
  • STEM CELLS
  •  141-153
  •  23 November 2010
Open access

Pluripotent stem cell‐derived retinal organoids for disease modeling and development of therapies

Kamil Kruczek, Anand Swaroop,
  • STEM CELLS
  •  1206-1215
  •  7 June 2020

Abstract

Retinal organoids offer a unique human model system to study mechanisms of disease pathogenesis and for developing therapies.
free access

Stem cell homing: From physiology to therapeutics

Jane L. Liesveld, Naman Sharma, Omar S. Aljitawi,
  • STEM CELLS
  •  1241-1253
  •  11 June 2020

Abstract

Steps in hematopoietic stem cell (HSC) homing and egress to and from marrow. Homing (left hand panel) occurs in the marrow sinusoidal endothelium. (1) Selectin-mediated braking or rolling occurs. Selectins involved include P-selectin which interacts with CD162 and SLex, and E-selectin which recognizes CD15, SLex, and CD162 on stem cells. (2) HSCs migrate on adhesion ligands presented by the vascular endothelium, so-called tethering. This involves CD49d-f, CD11b, and CD11c on the hematopoietic stem and progenitor cells and CD106/fibronectin, laminin, and ICAM-1 on endothelium as well as numerous other adhesion receptors and their ligands. (3) Changes in integrin conformation facilitate tight adhesion to the endothelial cell. (4) The stem cell then migrates through the endothelial cell cytoplasm or paracellularly. The cell will then migrate through matrix to reach the lodging destination. This is facilitated by CXCL12, c-kit ligand, cathepsins, and matrix metalloproteinases. Egress (right hand panel) involves: (1) Matrix transmigration in response to chemokine and growth factor effects. Matrix components involved include collagen IV, laminin, fibronectin, hyaluronic acid, and tenascin; among others. (2) Trans-endothelial migration, and (3) Cell release with disruption of receptor/ligand interactions such as those between between CXCL12 and CXCR4 or CD49d and CD106 (VCAM-1).
free access

Concise Review: Application of In Vitro Transcribed Messenger RNA for Cellular Engineering and Reprogramming: Progress and Challenges

Heidrun Steinle, Andreas Behring, Christian Schlensak, Hans Peter Wendel, Meltem Avci-Adali,
  • STEM CELLS
  •  68-79
  •  2 June 2016

Abstract

The exogenous delivery of synthetic messenger RNA (mRNA) into cells provides unlimited opportunities in cellular engineering without genomic integration.
Open access

Concise Review: Human Embryonic Stem Cells—What Have We Done? What Are We Doing? Where Are We Going?

Dusko Ilic, Caroline Ogilvie,
  • STEM CELLS
  •  17-25
  •  28 June 2016

Abstract

Ethical issues, the regulatory landscape, and the limited spectrum of diseases were all drawbacks of hESC lines that hiPSC did not have. It is to expect that in foreseeable future hiPSC will take over hESC in both research and clinical applications.

Latest news

More news >